CD BioGlyco, a biotechnology company offering a full range of glycobiology-related products, assays, custom synthesis and design to customers worldwide, now supports glycan separation and purification with advanced techniques.
Analysis of protein glycosylation is possible at multiple levels, including intact glycoproteins, glycopeptides, and released glycans. To effectively separate the different glycoforms of glycoproteins, an examination of the entire glycoprotein is challenging, especially for proteins with a variety of glycosylation sites. Assignment of specific glycan structures to different glycosylation sites is possible using site-specific information from glycopeptide analysis, which can identify the distribution of glycosylation at specific sites in proteins. The molecular composition of proteins is also important to understand.
Using chemical or enzymatic techniques, glycans can be released from proteins, and derivatization processes are then often used to improve the properties of the glycans for screening. Additionally, glycan enrichment should be performed when working with complex biological materials.
Backed by professional researchers and experimental instrumentation, CD BioGlyco now offers customers efficient separation, purification and analysis of glycans. Key techniques offered by CD BioGlyco include:
Capillary Electrophoresis (CE)
Based on CE technology, CD BioGlyco offers a high-level service for identifying the structure and linkage composition of unidentified glycans in samples.
Reversed Phase Liquid Chromatography (RPLC)
In glycomics, RPLC is a well-known and useful LC separation technique. It is compatible with electrospray ionization and provides repeatable results and good separation.
Hydrophilic interaction chromatography (HILIC)
A variety of HILIC separation techniques have been developed by CD BioGlyco to assist customers in solving separation issues related to strong polar pharmaceuticals and small biomolecules.
Porous Graphitized Carbon (PGC)
Glycans released from complex samples can be characterized by CD BioGlyco using PGC-LC-MS.
Lectin enrichment
Mass spectrometry can be used to enrich and detect glycoproteins that express specific glycans using lectin enrichment techniques developed by CD BioGlyco.
“With high-precision analytical equipment, professional teams dedicated to glycan separation and detection, and highly flexible service, we are confident to provide customers with characteristic and efficient detection solutions.” Said Anna, one of the representative speakers from CD BioGlyco.
To know more detailed information about the glycan separation and purification service offered by CD BioGlyco, please visit https://www.bioglyco.com/glycan-separation-and-purification-service.html.
